A simple in vitro technique to measure the release of endogenous dopamine and dihydroxyphenylacetic acid from striatal tissue using high performance liquid chromatography with electrochemical detection.
نویسندگان
چکیده
An easily constructed, inexpensive and simple system is described for the superfusion of neural tissue. To characterize the system, the dynamics of endogenous dopamine and dihydroxyphenylacetic acid release from striatal tissue were studied before and after exposure to potassium, amphetamine or cocaine.
منابع مشابه
In vivo effects of inorganic mercury (HgCl(2)) on striatal dopaminergic system.
In the present study, the effects of intrastriatal administration of different concentrations (40 microM, 400 microM, and 4 mM) of inorganic mercury (HgCl(2)) on the dopaminergic system of rat striatum were evaluated, using a microdialysis technique coupled to liquid chromatography-electrochemical detection. In previous studies, we discussed the effects of organic mercury (MeHg) administration ...
متن کاملA method of in vivo simultaneous measurements of dopamine, serotonin and their metabolites using intracerebral microdialysis system.
The present study demonstrated that it is possible to measure the endogenous extracellular dopamine (DA) and its metabolite dihydroxyphenylacetic acid (DOP AC), endogenous extracellular 5-hydroxytryptamine (5HT), its metabolites 5-hydroxyindoleacetic acid (5HIAA), 5-hydroxytryptophol (5HTOL) and its precursor 5-hydroxytryptophan (5HTP) simultaneously from the striatum of freely moving rats usin...
متن کاملTrans-striatal dialysis coupled to reverse phase high performance liquid chromatography with electrochemical detection: a new method for the study of the in vivo release of endogenous dopamine and metabolites.
A method for the estimation in rats of the in vivo release and metabolism of dopamine (DA) is described. The method is based on the dialysis principle and consists of inserting transversally in the striatum a thin (0.2 mm) dialysis tube (Amicon Vitafiber) which is then perfused with Ringer. The Ringer, flowing at a constant rate of 2 microliters/min in the dialysis tube, extracts low molecular ...
متن کاملStriatal dopamine in acute cerebral ischemia of stroke-resistant rats.
We devised the present experiments to assess the effects of ischemia on the production of dopamine in the caudate nucleus of spontaneously hypertensive stroke-resistant rats. Ringer's solution was continuously perfused at a rate of 10 microliters/min through 0.2-mm-diameter dialysis tubing implanted in the rat's caudate nucleus. After bilateral occlusion of the common carotid artery, perfusate ...
متن کاملDetermination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography
Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of neuroscience methods
دوره 11 1 شماره
صفحات -
تاریخ انتشار 1984